BT8251 Syllabus BIOCHEMISTRY Regulation 2017 Anna university

BT8251 Syllabus OBJECTIVE:

To enable students learn the fundamentals of Biochemical Processes and Biomolecules.

BT8251 Syllabus UNIT I INTRODUCTION TO BIOMOLECULES:

Basic principles of organic chemistry, role of carbon, types of functional groups, biomolecules, chemical nature of water, pH and biological buffers.

BT8251 Syllabus UNIT II STRUCTURE AND PROPERTIES OF IMPORTANT BIOMOLECULES:

Carbohydrates (mono, di, oligo & polysaccharides) mutarotation, glycosidic bond, reactions of monosaccharides and reducing sugars Starch, glycogen, cellulose and chitin. Proteoglycans, glycosaminoglycans. hyaluronic acid, chondroitin sulfate. Lipids: Fatty acids, glycerol, triacylglycerol, saponification, iodination, hydrogenation, phospholipids, glycolipids, sphingolipids. Inherited metabolic disorders of Lipid-metabolism-Tay-Saach’s disease, Niemann-Pick’s disease and Gaucher’s disease. Cholesterol, steroids, Bile acids and salts, Glucoand
Mineralo-corticosteroids. Aldosterone, cortisone and synthetic derivative-prednisolone. Androgens-testosterone, Estrogens- estrone, estradiol and progesterone. Prostaglandins and their functions. LDL, HDL and VLDL. Cardiovascular disease and correlation with circulating lipid and
lipoprotein concentration Amino Acids, Peptides, and Proteins.Classification based on side-chain properties. Structures, hierarchy of organization primary, secondary, tertiary and quaternary structures, glycoproteins, lipoproteins. Determination of primary structure. Nucleic acids: Purines, pyrimidines, nucleosides, nucleotides, Chargaff’s Rules. Base pairing, A-T and G-C, mRNA, rRNA and tRNA., Watson-Crick structure of DNA. reactions, properties, Tm and
hypochromicity, Measurement of DNA and RNA. Nucleoprotein complexes.

BT8251 Syllabus UNIT III METABOLISM CONCEPTS:

Functions of Proteins, Enzymes, introduction to biocatalysts, metabolic pathways, primary and secondary metabolites. Interconnection of pathways and metabolic regulation.

BT8251 Syllabus UNIT IV INTERMEDIARY METABOLISM AND REGULATION:

Glycolysis, TCA cycle, gluconeogenesis, pentose phosphate shunt, glyoxalate shunt, fatty acid synthesis and oxidation, reactions of amino acids, deamination, transamination and decarboxylation, urea cycle, Bioenergetics – High energy compounds, electronegative potential of compounds, respiratory chain, ATP cycle, calculation of ATP yield during oxidation of glucose and fatty acids.

BT8251 Syllabus UNIT V CASE STUDIES:

Case study on overproduction of primary and secondary metabolites – glutamic acid, threonine , lysine, methionine, isoleucine, propionic acid and ethanol.

BT8251 Syllabus OUTCOMES:

• To ensure students have a strong foundation in the structure and reactions of Biomolecules.
• For introduce them to metabolic pathways of the major biomolecules and relevance to clinical conditions.
• To correlate Biochemical processes with Biotechnology applications.

BT8251 Syllabus TEXT BOOKS

1. Lehninger Principles of Biochemistry 6th Edition by David L. Nelson, Michael M. Cox

2. Satyanarayana, U. and U. Chakerapani, “Biochemistry” 3rd Rev. Edition, Books & Allied (P) Ltd., 2006.

3. Rastogi, S.C. “Biochemistry” 2nd Edition, Tata McGraw-Hill, 2003.

4. Conn, E.E., etal., “Outlines of Biochemistry” 5th Edition, John Wiley & Sons, 1987.

5. Outlines of biochemistry, 5th Edition: By E E Conn, P K Stumpf, G Bruening and R Y Doi. pp 693. John Wiley and Sons, New York. 1987.

BT8251 Syllabus REFERENCES:

1. Berg, Jeremy M. et al. “Biochemsitry”, 6th Edition, W.H. Freeman & Co., 2006.

2. Murray, R.K., etal “Harper’s Illustrated Biochemistry”, 27th Edition, McGraw-Hill, 2006.

3. Voet, D. and Voet, J.G., “Biochemistry”, 3rd Edition, John Wiley & Sons Inc.,2004.

Subject Name	BIOCHEMISTRY
Subject Code	BT8251
Regulation	2017
File	PDF
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