BT8261 Syllabus BIOCHEMISTRY LABORATORY Regulation 2017 Anna University
BT8261 Syllabus AIM
- To learn and understand the principles behind the qualitative and quantitative estimation of biomolecules (proteins, carbohydrates, lipids, metabolites etc.,) and laboratory analysis of the same in the body fluids.
BT8261 Syllabus EXPERIMENTS
1. General guidelines for working in biochemistry lab (theory).
2. Units of volume, weight, density and concentration measurements and their range in biological measurements. Demonstration of proper use of volume and weight measurement devices.
3. Accuracy, precision, sensitivity and specificity (theory).
4. Preparation of buffer –titration of a weak acid and a weak base.
5. Qualitative tests for carbohydrates – distinguishing reducing from non-reducing sugars and keto from aldo sugars.
6. Quantitative method for amino acid estimation using ninhydrin – distinguishing amino from imino acid.
7. Protein estimation by Biuret and Lowry’s methods.
8. Protein estimation by Bradford and spectroscopic methods.
9. Extraction of lipids and analysis by TLC.
10. Estimation of nucleic acids by absorbance at 260 nm and hyperchromic effect (demo).
11. Enzymatic assay: phosphatase from potato.
12. Enzymatic assay: estimation of glucose by GOD-POD method after hydrolysis of starch with acid and specificity of the enzymatic method.
Equipment Needed for 20 Students
Autocalve 1
Hot Air Oven 1
Incubators 2
Light Microscopes 4
Incubator Shaker 1
Colorimeter 2
Laminar Flow Chamber 2
Glassware, Chemicals, Media as required
TOTAL: 60 PERIODS
BT8261 Syllabus TEXT BOOKS
1. Practical Biochemistry by R.C. Gupta and S. Bhargavan.
2. Introduction of Practical Biochemistry by David T. Phummer. (II Edition)
BT8261 Syllabus REFERENCES
1. Harpers Biochemistry Ed. R.K. Murray, D.K. Granner, P.A. Mayes and V.W.Rodwell, Appleton and Lange ,Stanford ,Conneticut.
2. Textbook of Biochemistry with clinical correlations. Ed. Thomas M. Devlin. Wiley Liss Publishers.
| Subject Name | BIOCHEMISTRY LABORATORY |
| Subject Code | BT8261 |
| Regulation | 2017 |
| File | |
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Semester 2 Anna University Regulation 2017 Syllabus.
Syllabus for sem 2 Anna University Regulation 2017.