BM8211 Syllabus BIOCHEMISTRY LABORATORY Regulation 2017 Anna University

BM8211 Syllabus OBJECTIVES:

To provide practice on:

• Estimation and quantification of bio molecules.
• Separation of macro molecules.
• Estimation and interpretation of biochemical parameter.

BM8211 Syllabus LIST OF EXPERIMENTS:

1. General guidelines for working and functional component of biochemistry lab

2. Preparation of solutions:

• Percentage solutions,
• Molar solutions,
• Normal solutions

3. Standardization of pH meter, preparation of buffers, emulsions.

4. Spectroscopy: Determination of absorption maxima (λmax) of a given solution

5. General tests for carbohydrates, proteins and lipids.

6. Identification of Blood Collection Tubes and Phlebotomy equipments

7. Preparation of serum and plasma from blood.

8. Estimation of Haemoglobin

9. Estimation of blood glucose.

10. Estimation of creatinine.

11. Estimation of urea.

12. Estimation of Uric acid

13. Estimation of cholesterol

14. Assay of SGOT/SGPT.

15. ELISA test

16. Separation of proteins by SDS electrophoresis(Demo)

17. Separation of amino acids by thin layer chromatography (Demo).

TOTAL: 60 PERIODS

BM8211 Syllabus OUTCOMES:

Upon completion of the course, students will be able to:

• Understand the Biochemistry laboratory functional components
• Understand the basics principle of preparation of buffers.
• Have a sound knowledge of qualitative test of different biomolecules.
• Understand the basics knowledge of Biochemical parameter and their interpretation in Blood sample.
• Have a sound knowledge of separation technology of proteins and aminoacids.

BM8211 Syllabus LAB REQUIREMENT FOR A BATCH OF 30 STUDENTS:

Requirement for a batch of 30 students

• Colorimeter 2 Nos
• Spectrophotometer 1 No.
• pH meter 1 No
• Weighing balance 1 No
• Refrigerator 1 No
• SDS gel electrophoresis 1 No
• TLC, ready TLC plates 1 No
• Wintrobe’s tube 2 Nos.
• Centrifuge Normal 1 No
• Microslides 2 packets
• Lancet 5 boxes
• Microscope 1 No
• Neubaur’s Chamber 2 Nos.
• Heparinized Syringe 1box
• Haemoglobinometer 1 No
• Elisa reader.1 nos
• Capillary tubes 1 box

 

Subject Name	BIOCHEMISTRY LABORATORY
Subject Code	BM8211
Regulation	2017
File	PDF
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Semester 2 Anna University Regulation 2017 Syllabus.

Syllabus for sem 2 Anna University Regulation 2017.